Mitochondrial Phosphoproteome Revealed by an Improved IMAC Method and MS/MS/MS
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چکیده
منابع مشابه
Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS.
IMAC in combination with mass spectrometry is a promising approach for global analysis of protein phosphorylation. Nevertheless this approach suffers from two shortcomings: inadequate efficiency of IMAC and poor fragmentation of phosphopeptides in the mass spectrometer. Here we report optimization of the IMAC procedure using (32)P-labeled tryptic peptides and development of MS/MS/MS (MS3) for i...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2007
ISSN: 1535-9476
DOI: 10.1074/mcp.m600218-mcp200